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| Product Code | mab90010-P |
|---|---|
| Clone | BA3R |
| Immunogen | beta-Actin N-terminal peptide-KLH conjugates |
| Target Specificity | Reacts with 42kDa native and denatured forms of beta-actin from human, mouse, rat, rabbit, and chicken. |
| Name | Beta-actin |
| Uniprot ID | P60709 |
| Gene ID | 60 |
| Product type | Primary antibodies, Loading controls |
|---|---|
| Clonality | Monoclonal antibody |
| Isotype | IgG2b |
| Produced in | Mouse |
| Species | Human, Mouse, Rat, Rabbit, Chicken |
| Labelling | None |
| Appearance | Liquid |
| Conc. / Activity | 1mg/ml |
| Form | Purified |
| Constituents | PBS 10mM, (pH 7.2) |
| Preservatives | NaN3 0.05% |
| Storage | Short term storage at +4°C (add 0.1% NaN3).For extended periods store in aliquots at -20°C.Antibodies are guaranteed for 6 month from date of receipt. |
| Applications | Western Blot, Immunofluorescence, Immunocytochemistry, Flow Cytometry, ELISA, Immunoprecipitation |
| Working dilutions | Optimal dilution should be determined by the end user. The following are guidelines only :WB(1:1000 - 1:5000)FC(0.1 - 1µg for 106^ cells)ICC/IF(10 µg/ml)IP(5 µg/ml) |
| Research areas | Cell metabolism |
* Deygas M et al. Nat Commun. 2018 Oct 31;9(1):4545. (WB, Hu)
- (mab90010-P)Anti-beta-actin antibody WB staining of cell lysates from (1) human, (2) mouse, (3) rat, (4) rabbit.">
- (mab90010-P)Overlay histogram showing HeLa cells stained with mab90010-P (red line). Cells were fixed and permeabilized before staining. Unlabelled cells correspond to blue line and isotype control to black line.">
- (mab90010-P)Anti-beta-actin antibody IF staining (green) of HeLa cells. Plasma membranes are labelled in red and nuclei in blue (DAPI).">
- (mab90010-P)Beta-actin IP using HeLa whole cell extract (5 µg of antibody for 0.5 mg of cell extract)">
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covalab关于单克隆抗体
在免疫反应过程中，抗原加工细胞将静止的B细胞活化为浆细胞，以分泌针对该抗原的免疫球蛋白。从多个参与免疫反应的浆细胞克隆中，可以通过普通的细胞培养技术将每个分离。一旦分离，该细胞及其后代就会产生相同亚型的相同免疫球蛋白。这些免疫球蛋白于是被称为单克隆抗体，并且对所需抗原的一个表位具有相同的亲和力和特异性。
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