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| Product Code | opr0028 |
|---|---|
| Uniprot ID | P22735 |
| Name | Transglutaminase-1 (TGase-1) |
| Appearance | White lyophilized solid |
|---|---|
| Constituents | The TG is lyophilized from 50 mM Tris-HCl pH 8.0, 10 mM Glutathion. |
| Storage | Store working aliquots at a minimum of -20°CAvoid repeated freezing and thawingDelivery at ambient temperature is possible |
| Description | His6-rhTG1 is based on the TGM1-allele from I.M.A.G.E.-clone IRAKp961M1628 isolated from human skin squamous cell carcinoma. It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MHHHHHHMDGPR. His6-rhTG1 is purified by IMAC to more than 90 % purity. |
| Produced in | Escherichia coli |
| Species | Human |
| Form | Recombinant |
| Gene ID | 7051 |
| Labelling | His-Tag |
| Preparation method | Add 10 mM Ca2+ to activate His6-rhTG1. |
| Molecular weight | 90 kDa |
| Principle | His6-rhTG1 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG1 may also be used for immunoprecipitation. |
| Purity | > 90 % (visually by SDS-PAGE) |
| Recommendations | Add the volume of water specified in the certificate of analysis under aliquotation to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be cooled on ice for short term storage. |
| Product type | Proteins |
| Applications | Immunoprecipitation, Functional Assay |
| Research areas | Transglutaminase |
品牌介绍
covalab关于单克隆抗体
在免疫反应过程中，抗原加工细胞将静止的B细胞活化为浆细胞，以分泌针对该抗原的免疫球蛋白。从多个参与免疫反应的浆细胞克隆中，可以通过普通的细胞培养技术将每个分离。一旦分离，该细胞及其后代就会产生相同亚型的相同免疫球蛋白。这些免疫球蛋白于是被称为单克隆抗体，并且对所需抗原的一个表位具有相同的亲和力和特异性。
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