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| Product Code | mab90001-P |
|---|---|
| Clone | HIS.H8 / EH158 |
| Immunogen | HHHHHH (6x His) synthetic peptide conjugated to KLH |
| Target Specificity | Reacts with the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag"s location in the fusion protein sequences (i.e.: reacts with N-terminal, C-terminal or internal 6x His-tags) |
| Name | poly-histidine |
| Product type | Primary antibodies, Tags |
|---|---|
| Clonality | Monoclonal antibody |
| Isotype | IgG2b |
| Produced in | Mouse |
| Labelling | None |
| Appearance | Liquid |
| Conc. / Activity | 1 mg/mL |
| Form | Purified |
| Constituents | PBS 10mM, (pH 7.2) |
| Preservatives | NaN3 0.05% |
| Storage | Short term storage at +4°C (add 0.1% NaN3).For extended periods store in aliquots at -20°C.Antibodies are guaranteed for 6 month from date of receipt. |
| Applications | Western Blot, Immunocytochemistry, ELISA, Immunoprecipitation |
| Working dilutions | Optimal dilution should be determined by the end user. The following are guidelines only : WB 1:1000 - 1:3000 ICC1:500 - 1:2000 |
* Ossareh-Nazari B et al. Ubiquitylation by the Ltn1 E3 ligase protects 60S ribosomes from starvation-induced selective autophagy. J Cell Biol. 2014 Mar 17,204(6):909-17.
- (mab90001-P)Anti-His Tag antibody ICC staining of His-tag fusion protein in 293 cells.Left hand panel: untransfected controlRight hand panel: transfected.Counterstained with DAPI (blue).">
- (mab90001-P)Anti-His Tag antibody WB staining of recombinant His-tagged Tgase-2 (1) 300 ng or (2) 30 ng. Antibody mab90001-P dilution 1/1000">
- (mab90001-P)Anti-His Tag antibody ICC staining of transfected human HEK293 cells. DAPI staining (blue).">
- (mab90001-P)Anti-His Tag antibody WB staining of various amounts of E. coli lysate containing a multi-epitope tagged protein">
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covalab关于单克隆抗体
在免疫反应过程中，抗原加工细胞将静止的B细胞活化为浆细胞，以分泌针对该抗原的免疫球蛋白。从多个参与免疫反应的浆细胞克隆中，可以通过普通的细胞培养技术将每个分离。一旦分离，该细胞及其后代就会产生相同亚型的相同免疫球蛋白。这些免疫球蛋白于是被称为单克隆抗体，并且对所需抗原的一个表位具有相同的亲和力和特异性。
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