| Product Code | pab72983 |
|---|---|
| Immunogen | Recombinant protein fragment containing a sequence corresponding to a region within amino acids 9 and 323 of GAPDH. |
| Target Specificity | Reacts with human GAPDH. Cross reacts with drosophila, mouse and rat protein. |
| Name | GAPDH |
| Uniprot ID | P04406 |
| Product type | Primary antibodies, Loading controls |
|---|---|
| Clonality | Polyclonal antibody |
| Produced in | Rabbit |
| Species | Human, Mouse, Rat, Drosophila melanogaster |
| Labelling | None |
| Appearance | Liquid |
| Form | Antigen affinity purified |
| Constituents | PBS, pH 7, 1% BSA, glycerol 20% |
| Preservatives | Thiomersal 0.01% |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Western Blot, IHC - Paraffin sections, Immunofluorescence, Immunocytochemistry |
| Working dilutions | Optimal dilution should be determined by the end user.The following are guidelines only :ICC (1:100 - 1:200) IF (1:100 - 1:200)IHC-P (10 µg/ml)WB (1:5000 - 1:20000) |

在免疫反应过程中,抗原加工细胞将静止的B细胞活化为浆细胞,以分泌针对该抗原的免疫球蛋白。从多个参与免疫反应的浆细胞克隆中,可以通过普通的细胞培养技术将每个分离。一旦分离,该细胞及其后代就会产生相同亚型的相同免疫球蛋白。这些免疫球蛋白于是被称为单克隆抗体,并且对所需抗原的一个表位具有相同的亲和力和特异性。



