| Product Code | mab50082 |
|---|---|
| Clone | 2D4A7 |
| Immunogen | Ni-NTA purified recombinant human GAPDH expressed in E. Coli strain BL21 (DE3) |
| Target Specificity | Reacts with human GADPH. Cross reacts with mouse, rat and rabbit protein. Since GAPDH is one of the most conserved proteins known, it is likely that the antibody is effective on other species also. |
| Name | Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) |
| Uniprot ID | P04406 |
| Product type | Primary antibodies, Loading controls |
|---|---|
| Clonality | Monoclonal antibody |
| Isotype | IgG1 / Kappa |
| Produced in | Mouse |
| Species | Human, Mouse, Rat, Rabbit |
| Labelling | None |
| Appearance | Liquid |
| Conc. / Activity | 0,75 mg/ml |
| Form | Purified (protein G) |
| Constituents | Tris-glycine, NaCl 150mM buffer |
| Preservatives | NaN3 0.05% |
| Storage | Short term storage at +4°C.For extended periods store in aliquots at -20°C.Antibodies are guaranteed for 6 month from date of receipt. |
| Applications | Western Blot, ELISA |
| Working dilutions | Optimal dilution should be determined by the end user. The following are guidelines only :- ICC : 1/2 000 to 1/20 000- IF : 1/2 000 to 1/20 000- WB : 1/20 000 |
| Research areas | Cell metabolism,Neurology |

在免疫反应过程中,抗原加工细胞将静止的B细胞活化为浆细胞,以分泌针对该抗原的免疫球蛋白。从多个参与免疫反应的浆细胞克隆中,可以通过普通的细胞培养技术将每个分离。一旦分离,该细胞及其后代就会产生相同亚型的相同免疫球蛋白。这些免疫球蛋白于是被称为单克隆抗体,并且对所需抗原的一个表位具有相同的亲和力和特异性。



