Appearance | White lyophilized solid |
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Constituents | The TG is lyophilised from 50mM NaH2PO4, 150 mM NaCl, pH8 and less than 0.1 mM Imidazole. Sample contains maltodextrin His6_rhTG2 is a Ca2+ dpt enzyme. |
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Storage | Store working aliquots at a minimum of -20°CAvoid repeated freezing and thawing |
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Description | His6-rhTG2 is based on the TGM2-allele from I.M.A.G.E.-clone IMAGp958L121020 isolated from neuroblastoma cells of the human brain. It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MAHHHHHHAEELV….His6-rhTG2 is produced in E. coli and purified by ion metal chelating chromatography to more than 90 % purity. |
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Produced in | Escherichia coli |
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Species | Human |
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Form | Recombinant |
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Gene ID | 7052 |
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Labelling | His-Tag |
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Preparation method | The Transglutaminase is activated with 10 mM Ca2+; due to the precipitation of Calcium Phosphate a buffer exchange (e. g. Tris-Buffer) prior to activation is highly recommended. |
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PDB ID | 1FAU |
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Molecular weight | 78 kDa |
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Principle | His6-rhTG2 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG2 may also be used for immunoprecipitation. |
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Purity | > 90 % |
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Recommendations | Add the desired volume of H2O to the vial of lyophilized powder. |
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Product type | Proteins |
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Applications | Immunoprecipitation, Functional Assay |
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Research areas | Transglutaminase |
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