| Product Code | opr0059 |
|---|---|
| Uniprot ID | P21980 |
| Name | Transglutaminase-2 (TGase-2) |
| Endotoxin level | Free |
| Appearance | Liquid |
|---|---|
| Constituents | 10 mM Tris-HCl pH 7.2, 150 mM NaCl, 0.5 mM EDTA, 0.5 mM DTT, 10% Glycerol. |
| Storage | Store working aliquots at a minimum of -20°CAvoid repeated freezing and thawing |
| Description | Endotoxin level: < 4.5 EU/mg, sterile filtered using 0.2 micron filter [Bacterial endotoxin level was determined at SGS Institute Fresenius using the kinetic - turbidimetric method]. |
| Produced in | Insect cells |
| Species | Human |
| Form | Recombinant |
| Gene ID | 7052 |
| Labelling | His-Tag |
| Preparation method | Add 10 mM Ca2+ to activate His6-rhTG2. |
| Molecular weight | 78 kDa |
| Principle | His6-rhTG2 catalyzes acyl transfer reactions from glutamine residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG2 may also be used for immunoprecipitation. This product is suitable for cell culture use. |
| Purity | No |
| Product type | Proteins |
| Applications | Functional Assay |
| Research areas | Transglutaminase |

在免疫反应过程中,抗原加工细胞将静止的B细胞活化为浆细胞,以分泌针对该抗原的免疫球蛋白。从多个参与免疫反应的浆细胞克隆中,可以通过普通的细胞培养技术将每个分离。一旦分离,该细胞及其后代就会产生相同亚型的相同免疫球蛋白。这些免疫球蛋白于是被称为单克隆抗体,并且对所需抗原的一个表位具有相同的亲和力和特异性。



